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- MBS Monoclonals
- Flag Tag Antibody
Product short description
Price:
442 EUR
Size:
100ug
Catalog no.:
GEN854112
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Concentration
N/A
Purification method
N/A
Clone
N/A
Other names
N/A
Immunoglobulin isotype
IgG1
Conjugation
Flag
Also known as
Flag Tag
French translation
anticorps
Clonality
Monoclonal
Category
Antibodies
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Tested applications:
ELISA (EIA), Western Blot (WB-Re)
Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Form/Appearance
Each vial contains 100 ug lyophilized IgG. Spin 2-3 minutes before open the tube, add 100 ul ddH2O to reconstitute the lyophilized powder for 1 hour at 4 degree and mix gently by inverting 5-6 times at room temperature.
Specificity and cross-reactivity
Anti-Flag Tag recognizes Flag and Flag-tagged fusion proteins.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
the antibody should be stored at -20 degrees Celsius. It is stable for one year in its original formulation.the antibody may be stored at 2 - 8 degrees Celsius for up to one month without any significant loss of activity. Freeze-thaw cycles of the diluted antibody should avoided in order to retain its full activity.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.An anti-flag tag (FLAG fusion protein) is use to detect a FLAG-tag, or FLAG octapeptide, or FLAG epitope that is a polypeptide protein tag that can be added to a protein using recombinant DNA. This FLAG-tags have the sequence DYKDDDDK motiv. These tags are very useful to do protein purification by affinity chromatography. Also separation of recombinant, overexpressed proteins from cell lysates is done by FLAG go HIS tags. FLAGS are also used in the isolation of protein complexes with multiple subunits, because its mild purification procedure tends not to disrupt such complexes. It has been used to enrich proteins of height purity and quality to see the 3D crystal structure with x-ray. Suitable for in vivo use in cells. For electrophorese protein detection rabbit polyclonals anti Flag conjugation are the most suited antibodies.
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