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- HIV reverse transcriptase (HIV-RT) - Biotinylated
Product short description
Price:
370 EUR
Size:
100ug
Catalog no.:
GEN395229
Product detailed description
Virus
hiv
Gene name
N/A
Gene name synonims
N/A
Concentration
N/A
Other gene names
N/A
Other names
N/A
Clone
AS35
Immunoglobulin isotype
IgG1
Category
Antibodies
Clonality
Monoclonal
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Goup
reverse transcription
Purification method
Protein A/G Chromatography
Also known as
HIV reverse transcriptase (HIV-RT)
Tested applications:
ELISA (EIA), Immunoprecipitation (IP)
Form/Appearance
Provided as a 0.2 m sterile-filtered solution in Ca++ & Mg++ free Dulbecco's PBS with 0.08% sodium azide.
Storage and shipping
Product should be stored the antibody should be stored at -20 degrees Celsius.. Aliquot to avoid freeze/thaw cycles
Species reactivity
n/a; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Description
Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
Gene
The human immunodeficiency virus (HIV) is a lentivirus (a subgroup of retrovirus) that causes HIV infection and over time acquired immunodeficiency syndrome(AIDS). AIDS is a condition in humans in which progressive failure of the immune system allows life-threatening opportunistic infections and cancers to thrive. Without treatment, average survival time after infection with HIV is estimated to be 9 to 11 years, depending on the HIV subtype. Infection with HIV occurs by the transfer of blood, semen, vaginal fluid, pre-ejaculate, or breast milk. Within these bodily fluids, HIV is present as both free virus particles and virus within infected immune cells. recombinant HIV 1 and 2 gag gene proteins p24, p17, p55 immunodominant epitopes and envelope glycoproteins, gp120 are used for production of diagnostic detection antibodies.
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