Involucrin

354 EUR

100ug

GEN438424

N/A

N/A

N/A

IVL

N/A

SPM259

Monoclonal

Antibodies

Involucrin

IgG1, kappa

IVL; IVL; N/A; N/A

Mouse (Mus musculus)

Mnoclonal antibodies

involucrin; Involucrin; involucrin; involucrin; N/A

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blot (WB), Immunohistology (IH)

Store the antibody at +4C. Temperature variations in the range of 1 to 7 degrees Celsius are tolerable. We recommend NOT to freeze the antibody. Antibody is stable for 24 months.

Human (Homo sapiens), Gorilla, Owl monkey, Pig, Dog. Does not react with Mouse (Mus musculus).; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.