Mouse Anti-Melanoma Associated Antigen

343 EUR

1 mililiter

GEN570288

N/A

N/A

N/A

N/A

N/A

IgG1

NKI-C3

Antibodies

Monoclonal

Mus musculus

Mnoclonal antibodies

Mouse (Mus musculus)

Melanoma Associated Antigen

melanoma-associated antigen; N/A

Each vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.

ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin)

Store the antibody at +4 degrees Celsius., or in small aliquots the antibody should be stored at -20 degrees Celsius..

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antigens are peptides or recombinant or native dependent on the production method.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

NKI/C-3 is a MAb which is directed anti a predominantly intracellular protein of Human melanoma cells. The antigen is abundantly present in malignant melanomas, nevocellular nevi, and neuroendocrine tumors, whereas it has not been detectable in most other tissues. The antigen recognized by NKI/C-3 is a glycoprotein which appears as a broad band in SDS-PAGE. The antigen was shown to consist of a single protein backbone to which two or three.V-linked glycans were added cotranslationally. Extensive further heterogeneity was geneRated in the Golgi compartment and was shown to be dependent on the presence of complex type sugars. Although the antigen is associated with melanomas, it was not codistributed with the tyrosinase activity associated with melanogenesis. The antigen did show codistribution with Cathepsin D, which is a marker for lysosomal functions. NKI/C3 originally was described as a marker for melanoma. Recently, it resurfaced as a marker to sepaRate cellular neurothekeoma from other dermal tumors in the differential diagnosis.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.