TNF-RII, Mouse, mAb Biotin

531 EUR

50ug

GEN584379

N/A

N/A

N/A

N/A

IgG2a

TNF-RII

TNF-RII

Antibodies

Monoclonal

Mus musculus

Biotinylated

Mnoclonal antibodies

Mouse (Mus musculus)

TNFRSF1B; TNF-RII/TNFRSF1B; TNF-RII/TNFRSF1B; N/A

F, Flow Cytometry (FC/FACS), FS, Immuno Assay (IA), Immunoprecipitaion (IP), Western Blot (WB)

0.5 ml (100 ug/ml) 0.2 um filtered biotinylated antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.

N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

tumor necrosis factor receptor type two, partial; Tumor necrosis factor receptor type two; tumor necrosis factor receptor superfamily member 1B; tumor necrosis factor receptor superfamily, member 1B

N/A; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Product should be stored at Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable. Under recommended storage conditions, product is stable for one year.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Tumor necrosis factor (TNFa, tumor necrosis factor alpha, TNFα, cachexin, or cachectin) is a cell signaling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. TNFb or TNF beta also bin on TNF receptors for Th1 activation.