Microphthalmia (Transcription Factor, MiTF) (BSA & Azide Free)

707 EUR

100ul

GEN600026

N/A

3F276

IgG1,k

Antibodies

Monoclonal

Microphthalmia

Mnoclonal antibodies

Mitf; Mitf; N/A; N/A

Mitf; Mitf; N/A; N/A

Mitf; Mitf; N/A; N/A

Mouse (Mus musculus)

Human (Homo sapiens)

Immunohistochemistry (IHC), Gel Shift Assay (GS/EMSA)

Affinity Purified by Protein G affinity chromatography.

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See M3891-01

Canine, Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

microphthalmia associated transcription factor; Microphthalmia-associated transcription factor; microphthalmia-associated transcription factor; microphthalmia-associated transcription factor; N/A

It does not cross-react with other b-HLH-ZIP factors by DNA mobility shift assay.1 it reacts with both melanocytic and nonmelanocytic isoforms of Mi. Species Crossreactivity: human and canine. Does not react with mouse and rat.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze atStore the antibody at -20 degrees Celsius. or colder. Aliquots are stable for at least 12 months atStore the antibody at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Sodium azide NaN3 is often used to prevent antibody degradation in 0,02% W/V concentrations. MBS Monoclonals supplies azide free antibodies on request.Recently MBS Monoclonals made this Microphthalmia (Transcription Factor, MiTF) (BSA ) available without azide. It will stick less and is more suitable for ELISA or in vivo studies. However you need to store the @VV@ frozen and ship on dry ice.Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.The activation of transcription factor subunits is the first step of gene expression, in which a particular segment of DNA is copied into RNA (mRNA) by the enzyme RNA polymerases. Transcription factors, unites and elongations can be RNA and DNA nucleic acids, base pairs of nucleotides . Converting from DNA to RNA is made by enzymatic reactions. During transcription, a DNA sequence is read by an RNA polymerase, which produces a complementary, anti-parallel RNA strand called a primary transcript. Transcriptions are key functions in signal transduction pathways. Signaling ligand binding transcription factors play an important role in transduction cascades.