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- MBS Monoclonals
- CD134 Ligand (CD134L, OX40L, CD252) (FITC)
Product short description
Price:
691 EUR
Size:
120 Tests
Catalog no.:
GEN600114
Product detailed description
Concentration
N/A
Immunoglobulin isotype
IgG1
Clone
7H146
Category
Antibodies
Clonality
Monoclonal
Also known as
CD134 Ligand
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Source organism
Human (Homo sapiens)
Other gene names
TNFSF4; N/A; N/A; N/A
Gene name synonims
TNFSF4; N/A; N/A; N/A
Gene name
TNFSF4; N/A; N/A; N/A
Tested applications:
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
Purification method
Affinity Purified by Protein A affinity chromatography.
Other names
CD134(OX40)-ligand; N/A; tumor necrosis factor ligand superfamily member 4; CD134(OX40)-ligand; N/A; N/A
Description
FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Form/Appearance
Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with FITC. Molar Ratio: 8.1 FITC /IgG.
Specificity and cross-reactivity
Binds to CD134L on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage, For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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