Product short description
Product detailed description
Gene name synonims
Other gene names
Mouse (Mus musculus)
Human (Homo sapiens)
Also known as
Complement C5b Neo-epitope
Affinity Purified by Protein A affinity chromatography.
Supplied as a lyophilized powder. Reconstitute with 1ml sterile dH2O.
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Bovine, Goat, Human (Homo sapiens), Monkey, Porcine; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Specificity and cross-reactivity
Recognizes human C5b-9 complexes via activated C5, especially in different forms of glomerulonephritis. Does not react with native C5 (See C7850-23). Normal fresh and activated serum subjected to SDS-PAGE Western Blot (nonreduced), visualizes reactivity with the SDS-denatured C5 (200kD) abd C5b (185kD), the latter together with a faint cleavage product (60kD) only after activation.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Lyophilized powder may be stored the antibody should be stored at -20 degrees Celsius.. Stable for 12 months the antibody should be stored at -20 degrees Celsius.. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Reconstituted product is stable for 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer.