V5 epitope tag (Pk-tag, paramyxovirus, SV5)

442 EUR

100ug

GEN601200

N/A

N/A

N/A

N/A

N/A

9A208

IgG2a

Antibodies

Monoclonal

V5 epitope tag

Mnoclonal antibodies

Mouse (Mus musculus)

Supplied as a liquid in 0.09% sodium azide.

Affinity Purified by Protein G affinity chromatography.

ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Radioimmunoassay (RIA)

N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes recognizes a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. (V5-Tag). Detects recombinant proteins including transmembrane and secreted proteins, which have been tagged with this epitope. Usually, a 14 amino acid tag has been added to the recombinant proteins, although a smaller epitope of 9 amino acids (that as a peptide inhibit the binding of the monoclonal antibody to its native protein) has also been successfully used. The 14 amino acid epitope is; gly lys pro ile pro asn pro leu leu gly leu asp ser thr.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.