MHC Class 1, H2, Dk (Biotin)

591 EUR

500ug

GEN601209

N/A

N/A

N/A

N/A

N/A

IgG2a,k

Purified

15-5-5.3

Antibodies

Monoclonal

Biotinylated

MHC Class 1, H2, Dk

Mnoclonal antibodies

Mouse (Mus musculus)

Mouse (Mus musculus)

Flow Cytometry (FC/FACS)

Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Reacts with the H-2Dk class I alloantigen. It crossreacts with H-2Kd and with cells from mice with the H-2f haplotype. Does not react with other (e.g., a, b, p, q, r, s) haplotypes.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.