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- MBS Monoclonals
- CD229 (Ly-9) (FITC)
Product short description
Price:
696 EUR
Size:
100ug
Catalog no.:
GEN601857
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Concentration
N/A
Other gene names
N/A
Immunoglobulin isotype
IgG1
Also known as
CD229
Clone
3H1998
Category
Antibodies
Clonality
Monoclonal
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Source organism
Human (Homo sapiens)
Host organism
Mouse (Mus musculus)
Tested applications:
Flow Cytometry (FC/FACS)
Other names
cytochrome oxidase subunit I; N/A
Storage and shipping
Store the antibody at -20 degrees Celsius.
Purification method
Affinity Purified by Protein G affinity chromatography.
Form/Appearance
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Species reactivity
Human (Homo sapiens), Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Recognizes the human CD229 cell surface antigen, a 100-120kD cell surface glycoprotein that is the homologue of murine Ly-9.CD229 is a member of the immunogoblin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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