M13, fd, F1 Bacteriophages, Filamentous (HRP)

591 EUR

0.75 ml

GEN602100

N/A

N/A

N/A

N/A

N/A

5F167

IgG2b

E. coli

Antibodies

Monoclonal

HRP Conjugated

ELISA (EL/EIA)

Mnoclonal antibodies

Mouse (Mus musculus)

M13, fd, F1 Bacteriophages, Filamentous

Affinity Purified by Protein A affinity chromatography.

Supplied as a liquid with 0.01% thimersal. Labeled with Horseradish Peroxidase (HRP).

E Coli; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Recognizes an epitope on pVIII (phage coat protein) covering the N-terminal region of g8p AEGDDPAKAAFDSLQASAT.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.

M13 bacteriophage is a virus that infects the bacterium Escherichia coli. It is composed of a circular single-stranded DNA molecule encased in a thin flexible tube made up of about 2700 copies of a single protein called P8, the major coat protein. The ends of the tube are capped with minor coat proteins. Infection starts when the minor coat protein P3 attaches to the receptor at the tip of the F pilus of the bacterium. Infection with M13 is not lethal; however, the infection causes turbid plaques in E. coli because infected bacteria grow more slowly than the surrounding uninfected bacteria. It engages in a viral lifestyle known as a chronic infection which is neither lytic or temperate. However a decrease in the rate of cell growth is seen in the infected cells. M13 plasmids are used for many recombinant DNA processes, and the virus has also been studied for its uses in nanostructures and nanotechnology.