TLR4/MD-2 Complex (Toll Like Receptor 4/MD-2) (Biotin)

619 EUR

50ug

GEN602192

N/A

IgG2a

6A641

Antibodies

Monoclonal

Biotinylated

TLR4/MD-2 Complex

TLR4; N/A; N/A; N/A

TLR4; N/A; N/A; N/A

TLR4; N/A; N/A; N/A

Mnoclonal antibodies

Mouse (Mus musculus)

Affinity Purified IgG

Rat (Rattus norvegicus)

toll-like receptor 4; Toll-like receptor 4; toll-like receptor 4; N/A; N/A

Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA. Conjugated to Biotin.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Reacts with the Toll-like receptor 4 (TLR4, CD284). Reacts preferentially, especially in Flow Cytometry, with mouse TLR4 that is associated with MD-2. This antibody is a TLR4 function-blocking antibody that is useful for studies on the role of TLR4 as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to coprecipitate MD-2 (30kD) with TLR4 (100kD).; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.