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- c-erbB2, p185 (HER2, neu Receptor) (FITC)
Product short description
Price:
685 EUR
Size:
100 Tests
Catalog no.:
GEN602582
Product detailed description
Gene name
N/A
Concentration
N/A
Gene name synonims
N/A
Other gene names
N/A
Other names
N/A
Immunoglobulin isotype
IgG1
Clone
2B2.147
Category
Antibodies
Clonality
Monoclonal
Also known as
c-erbB2, p185
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Source organism
Human (Homo sapiens)
Host organism
Mouse (Mus musculus)
Tested applications:
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Purification method
Highly 95% prior to addition of stabilizer and preservative, as determined by SDS-PAGE.
Form/Appearance
Supplied as a liquid in 50mM Tris, 100mM sodium chloride, pH 7.4, 1% BSA, 0.02% sodium azide. Conjugated to FITC.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Recognizes human p185 HER-2.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Description
The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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