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- MBS Monoclonals
- CD257 (Biotin)
Product short description
Price:
691 EUR
Size:
100ug
Catalog no.:
GEN603154
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Concentration
N/A
Other gene names
N/A
Also known as
CD257
Clone
7H149
Immunoglobulin isotype
IgG1,k
Category
Antibodies
Clonality
Monoclonal
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Source organism
Human (Homo sapiens)
Host organism
Mouse (Mus musculus)
Other names
cytochrome oxidase subunit I; N/A
Tested applications:
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
Purification method
Affinity Purified by Protein A affinity chromatography.
Form/Appearance
Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Specificity and cross-reactivity
Binds to CD257 in EIA and blocks binding of recombinant in Flow cytometry, and blocks binding of recombinant CD257-muCD8 to receptors on Raji cell surface in Flow cytometry.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage, For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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