MCAM (Melanoma Cell Adhesion Molecule) (Biotin)

625 EUR

500ug

GEN603289

N/A

MCAM

cell

5K96

IgG2b,k

Chicken

Purified.

Antibodies

Monoclonal

Biotinylated

Mnoclonal antibodies

Mouse (Mus musculus)

MCAM; MCAM; CD146; MUC18; MUC18

MCAM; MCAM; CD146; MUC18; MUC18

MCAM; MCAM; CD146; MUC18; MUC18

Supplied as a liquid in PBS, 0.1% sodium azide. Labeled with Biotin.

Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Chicken; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Recognizes chicken MCAM, Mr 98kD (protein core ~84kD).; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

cell adhesion molecules play a role in cell growth and activation and are often identified by WB or ELISA as in the MCAM (Melanoma Adhesion Molecule) ( ).For cells, cell lines and tissues in culture till half confluency.Whole adhesion and interacting molecules are  present in lysates used as reference for ELISA quantification of these molecules and their subunits.

Melanoma cell adhesion molecule; Cell surface glycoprotein MUC18; cell surface glycoprotein MUC18; Gicerin; OTTHUMP00000232151; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; melanoma cell adhesion molecule; Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50?glycerol, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.