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- Histone H2A.X, phosphorylated (S139) (Biotin) (H2A histone family member X, H2A.FX, H2A/X, H2AFX, H2AX)
Product short description
Price:
829 EUR
Size:
100ug
Catalog no.:
GEN603432
Product detailed description
Concentration
N/A
Immunoglobulin isotype
IgG1
Clone
2T14
Category
Antibodies
Clonality
Monoclonal
Conjugation
Biotinylated
Also known as
Histone H2A.X
Tested applications:
Western Blot (WB)
Source organism
Human (Homo sapiens)
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Other gene names
H2AFX; H2AFX; H2AX; H2A.X; H2A/X; H2AX
Gene name synonims
H2AFX; H2AFX; H2AX; H2A.X; H2A/X; H2AX
Gene name
H2AFX; H2AFX; H2AX; H2A.X; H2A/X; H2AX
Purification method
Affinity Purified by Protein G affinity chromatography.
Other names
histone H2A.x; Histone H2A.x; histone H2A.x; H2AX histone; OTTHUMP00000232149; H2A histone family, member X; N/A
Form/Appearance
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide, before the addition of glycerol to 30%. Labeled with Biotin. Biotin
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage, For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Specificity and cross-reactivity
Recognizes human Histone H2A.X phosphorylated at Ser139, Mr 14kD. Species sequence homology: A broad species crossreactivity is expected based on conservation of sequence homology. The immunizing sequence has 8 identical amino acids in yeast and mouse.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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