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- Cytokeratin 5,8 (Biotin)
Product short description
Price:
663 EUR
Size:
0.25 ml
Catalog no.:
GEN607637
Product detailed description
Concentration
N/A
Immunoglobulin isotype
IgG1
Clone
5F173
Category
Antibodies
Clonality
Monoclonal
Conjugation
Biotinylated
Also known as
Cytokeratin 5,8
Source organism
Human (Homo sapiens)
Subcategory
Mnoclonal antibodies
Host organism
Mouse (Mus musculus)
Other gene names
Krt8; Krt8; CYKER; Krt2-8; Krt2-8
Gene name synonims
Krt8; Krt8; CYKER; Krt2-8; Krt2-8
Gene name
Krt8; Krt8; CYKER; Krt2-8; Krt2-8
Form/Appearance
Supplied as a liquid. Conjugated to Biotin.
Purification method
Affinity Purified by Protein A affinity chromatography.
Tested applications:
ELISA (EL/EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Species reactivity
Bovine, Human (Homo sapiens), Porcine; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Other names
cytokeratin 8 polypeptide; Keratin, type II cytoskeletal 8; keratin, type II cytoskeletal 8; K8; CK-8; keratin-8; cytokeratin-8; cytokeratin endo A; type-II keratin Kb8; keratin complex 2, basic, gene 8; keratin 8; Cytokeratin endo A; Cytokeratin-8; CK-8; Keratin-8; K8; Type-II keratin Kb8
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Specificity and cross-reactivity
Reactivities on Cultured MCF-7, RT 112, HT-29, Detroit 562, RPMI 2650. Cell lines (tested so far) SSC-12, bovine BMGE+H, BMGE-H, MDBK. Represents an excellent marker for distinguishing carcinomas from all non-epithelial tumors. Specifically reacts with cytokeratins 5 and 8 present in nearly all epithelia. Polypeptide reacting; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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