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- MBS Monoclonals
- IgE, Heavy Chain (FITC)
Product short description
Price:
647 EUR
Size:
500ug
Catalog no.:
GEN609201
Product detailed description
Additional isotype
IgE
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Concentration
N/A
Other names
N/A
Immunoglobulin isotype
IgG1
Clone
3H2284
Clonality
Monoclonal
Category
Antibodies
Also known as
IgE, Heavy Chain
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Source organism
Mouse (Mus musculus)
Host organism
Rat (Rattus norvegicus)
Tested applications:
Flow Cytometry (FC/FACS)
Purification method
Affinity Purified by affinity chromatography.
Form/Appearance
Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol. Labeled with fluorescein isothiocyanate (FITC).
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Recognizes mouse IgE. Does not crossreact with other immunoglobulin classes or subclasses. Does not crossreact with rat IgE.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor.
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