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- MBS Monoclonals
- CD3, epsilon (Early T Cell Marker) (Biotin)
Product short description
Price:
525 EUR
Size:
500ug
Catalog no.:
GEN609769
Product detailed description
Concentration
N/A
Clone
5K4
Tissue
cell
Immunoglobulin isotype
IgG2c,k
Purification method
Purified
Category
Antibodies
Clonality
Monoclonal
Also known as
CD3, epsilon
Conjugation
Biotinylated
Gene name
cd3e; N/A; N/A; N/A
Gene name synonims
cd3e; N/A; N/A; N/A
Other gene names
cd3e; N/A; N/A; N/A
Subcategory
Mnoclonal antibodies
Source organism
Mouse (Mus musculus)
Host organism
Rat (Rattus norvegicus)
Other names
CD3 epsilon; N/A; CD3 epsilon; N/A; N/A
Description
For cells, cell lines and tissues in culture till half confluency.
Form/Appearance
Supplied as a liquid in PBS, pH 7.4, sodium azide. Conjugated to Biotin.
Tested applications:
Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Mouse CD3e (Mr 25kD). Recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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