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- LAG-3 (Lymphocyte Activation Gene-3, FDC Protein, CD223) (FITC)
Product short description
Price:
851 EUR
Size:
100ug
Catalog no.:
GEN609777
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Other gene names
N/A
Immunoglobulin isotype
IgG1
Clone
6D65
Also known as
LAG-3
Category
Antibodies
Tissue
lymphocyte
Clonality
Monoclonal
Conjugation
Anti-FITC Antibody
Other names
LAG-3 protein; N/A
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Source organism
Human (Homo sapiens)
Tested applications:
Flow Cytometry (FC/FACS)
Concentration
1 miligram per 1 mililiter
Purification method
Affinity Purified by Protein A sepharose chromatography. (>95% by SDS-PAGE).
Form/Appearance
Supplied as a liquid in PBS, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Description
cDNA genes are a locus (or region) of DNA for functional transcript RNA or protein. An ELISA is used to detect the expressed protein in biological fluids, serum, saliva.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Recognizes the 30aa extra-loop of the first N-terminal D1 domain of human LAG-3.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
Properties
This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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