MHC Class 2, I-A (Biotin)

520 EUR

100 Tests

GEN631564

N/A

IgG1

3H440

Antibodies

Monoclonal

Biotinylated

Purified IgG.

MHC Class 2, I-A

Mnoclonal antibodies

Mouse (Mus musculus)

Rat (Rattus norvegicus)

orla-uaa; N/A; N/A; N/A

orla-uaa; N/A; N/A; N/A

orla-uaa; N/A; N/A; N/A

Flow Cytometry (FC/FACS)

Store the antibody at -20 degrees Celsius.

Mhc class I A; N/A; Mhc class I A; N/A; N/A

Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Purified IgG conjugated to Biotin - lyophilized in PBS with 1% BSA. The addition of 0.1% Sodium azide is recommended after reconstitution. Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Recognizes a monomorphic determinant of the rat Ia antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells (2, 3 and 4). The antibody cross-reacts with certain mouse strains (MHC haplotypes k and s) and analysis of recombinant strains showed that the determinants mapped to the I-A region of the mouse MHC (1,2). Species Crossreactivity: Does not recognize BDIX rat strain (5.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.