Vimentin, Stem Cell (VIM, FLJ36605, OTTHUMP00000019224)

669 EUR

100ug

GEN632146

N/A

IgM

9F512

Antibodies

cell, stem

Monoclonal

Vimentin, Stem Cell

Mnoclonal antibodies

Mouse (Mus musculus)

Human (Homo sapiens)

VIM; VIM; FLJ36605; N/A

VIM; VIM; FLJ36605; N/A

VIM; VIM; FLJ36605; N/A

15mM Sodium Azide; PBS. pH 7.4

Affinity Purified by immunoaffinity chromatography.

For cells, cell lines and tissues in culture till half confluency.

Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

vimentin; Vimentin; vimentin; OTTHUMP00000019224; OTTHUMP00000019225; vimentin; N/A

Chicken, Human (Homo sapiens), Mouse (Mus musculus), Porcine, Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Species Crossreactivity: Crossreacts with Human, Mouse, Rat, Chicken and porcine.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 3 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.