Threonine, Phosphorylated (Biotin)

685 EUR

100ul

GEN632418

N/A

N/A

N/A

N/A

IgG

11C156

Purified

Threonine

Monoclonal

Antibodies

Biotinylated

Mnoclonal antibodies

Mouse (Mus musculus)

ELISA (EL/EIA), Western Blot (WB)

threonine dehydratase, biosynthetic; N/A

Supplied as a lyophilized powder from PBS, 0.2% BSA, 0.05% sodium azide. Labeled with Biotin. Reconstitute with 100ul sterile PBS.

N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Recognizes phosphorylated threonine both as free amino acid or when conjugated to carriers such as BSA or KLH. Does not react with nonphosphorylated threonine, phosphorylated tyrosine or serine, AMP, or ATP.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Lyophilized powder may be stored the antibody should be stored at -20 degrees Celsius.. Stable for 12 months the antibody should be stored at -20 degrees Celsius.. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Reconstituted product is stable for 12 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer.