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- CD3, epsilon (Early T Cell Marker) (PE/CY5)
Product short description
Price:
486 EUR
Size:
100ug
Catalog no.:
GEN632552
Product detailed description
Concentration
N/A
Tissue
cell
Conjugation
Cy5.5
Immunoglobulin isotype
IgG,k
Host organism
Hamster
Clone
145-2C11
Purification method
Purified
Category
Antibodies
Clonality
Monoclonal
Also known as
CD3, epsilon
Other gene names
cd3e; N/A; N/A; N/A
Gene name synonims
cd3e; N/A; N/A; N/A
Gene name
cd3e; N/A; N/A; N/A
Subcategory
Mnoclonal antibodies
Source organism
Mouse (Mus musculus)
Other names
CD3 epsilon; N/A; CD3 epsilon; N/A; N/A
Description
For cells, cell lines and tissues in culture till half confluency.
Tested applications:
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Form/Appearance
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide and a stabilizing agent. Conjugated to PE/CY5.
Properties
Cy5 antibodies are excited by the 650-nanometer wave of an argon laser and detected at a 670-nanometer captor.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Specificity and cross-reactivity
Mouse CD3e (Mr 25kD). Recognizes an epitope on the 25kD epsilon chain of the CD3/TCR complex.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer. PE/CY5 combination conjugate is used as a third fluorochrome in multicolor flow cytometry applications when only a single 488nm excitation source is available. Antibodies are covalently coupled using heterobifunctional linking reagents, resulting in an optimal conjugation format for flow cytometry.
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