TCR, V beta 3 (T Cell Receptor) (Biotin)

630 EUR

50ug

GEN633157

N/A

N/A

N/A

N/A

cell

5C95

IgG2a

Antibodies

Monoclonal

Biotinylated

TCR, V beta 3

TCR, partial; N/A

Mnoclonal antibodies

Human (Homo sapiens)

Mouse (Mus musculus)

Flow Cytometry (FC/FACS)

Affinity Purified by Protein A affinity chromatography. Purity was 95% immunoglobulin by SDS-PAGE.

Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, before the addition of glycerol to 40%. Conjugated with NHS-Biotin.

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Specific for the human Vb3 TCR gene product and reacts with 1-10% of T cells in normal human blood. Antibody is mitogenic for T cells expressing TCR V b3.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

For long-term storage, aliquot and For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

For cells, cell lines and tissues in culture till half confluency.The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.