Product short description
Price:
542 EUR
Size:
500ug
Catalog no.:
GEN633370
Product detailed description
Gene name
N/A
Gene name synonims
N/A
Concentration
N/A
Other gene names
N/A
Other names
N/A
Clone
5K47
Immunoglobulin isotype
IgG2a,k
Purification method
Purified
Category
Antibodies
Clonality
Monoclonal
Conjugation
Biotinylated
Also known as
MHC Class 1, H2, Dd
Subcategory
Mnoclonal antibodies
Source organism
Mouse (Mus musculus)
Host organism
Mouse (Mus musculus)
Tested applications:
Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Form/Appearance
Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Specificity and cross-reactivity
Reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with b2-microglobulin. Does not react with H-2Dd alpha chains synthesized in vitro. Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H-2s haplotypes has been observed by flow cytometric analysis. Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed. Has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.