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- 5-Hydroxymethylcytosine (5hmC, 5'OH methyl cytosine)
Product short description
Price:
730 EUR
Size:
100ul
Catalog no.:
GEN643168
Product detailed description
Concentration
N/A
Immunoglobulin isotype
IgG1k
Clone
HMC-MA01
Category
Antibodies
Clonality
Monoclonal
Subcategory
Mnoclonal antibodies
Host organism
Rat (Rattus norvegicus)
Also known as
5-Hydroxymethylcytosine
Other gene names
hmces; N/A; im:7137789; zgc:165500; N/A
Gene name synonims
hmces; N/A; im:7137789; zgc:165500; N/A
Gene name
hmces; N/A; im:7137789; zgc:165500; N/A
Purification method
Affinity Purified by Protein G affinity chromatography.
Tested applications:
Immunoprecipitation (IP), Immunocytochemistry (ICC), Gel Shift Assay (GS/EMSA)
Form/Appearance
Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM NaCl, 0.05% sodium azide.
Species reactivity
N/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Other names
5-hydroxymethylcytosine (hmC) binding, ES cell-specific; N/A; 5-hydroxymethylcytosine (hmC) binding, ES cell-specific; 5-hydroxymethylcytosine (hmC) binding, ES cell-specific; 5-hydroxymethylcytosine (hmC) binding, ES cell-specific; N/A
Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Specificity and cross-reactivity
Detects 5-hydroxymethyl cytosine, but not 5-methylcytosine or unmethylated cytosine. Non-species specific antibody against modified DNA. Expected to detect 5-hydroxymethylcytosine in all species.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Description
A methyl group is an alkyl derived from methane, containing one carbon atom bonded to three hydrogen atoms — CH3. In formulas, the group is often abbreviated Me. Such hydrocarbon groups occur in many organic compounds. It is a very stable group in most molecules. While the methyl group is usually part of a larger molecule, it can be found on its own in any of three forms anion, cation or radical. The anion has eight valence electrons, the radical seven and the cation six. All three forms are highly reactive and rarely observed,
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