RLA, DR (Biotin)

619 EUR

500ug

GEN633122

N/A

N/A

N/A

N/A

N/A

5K114

RLA, DR

IgG2b,k

Purified

Antibodies

Monoclonal

Biotinylated

Mnoclonal antibodies

Mouse (Mus musculus)

Rabbit (Oryctolagus cuniculus)

Supplied as a liquid in PBS, 0.1% sodium azide. Conjugated to Biotin.

Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)

Rabbit (Oryctolagus cuniculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Store the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store the antibody should be stored at -20 degrees Celsius. or colder. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes rabbit RLA-DR chain (Mr ~35kD). Reacts with RLA-DR-transfected cells, but not with RLA-DQ-transfected cells and, in normal adult rabbits, 50-60% of peripheral blood leukocytes and splenocytes. It has been reported that the antibody stains: lymphocytes in the thymic medulla and some cells in the cortex; periportal cells in the liver; and synovial lining cell (i.e., infiltrating macrophages) in inflamed synovium.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.