Mouse Anti-Lactoferrin

359 EUR

200ug

GEN570346

4C5

N/A

N/A

IgG1

Antibodies

Lactoferrin

Mus musculus

Monoclonal antibody

Mnoclonal antibodies

Mouse (Mus musculus)

Flow Cytometry (FC/FACS), Indirect Immunofluorescence (IF)

LTF; LTF; LF; HLF2; GIG12; HEL110; GIG12; LF; Lactoferrin; Lfcin-H

LTF; LTF; LF; HLF2; GIG12; HEL110; GIG12; LF; Lactoferrin; Lfcin-H

LTF; LTF; LF; HLF2; GIG12; HEL110; GIG12; LF; Lactoferrin; Lfcin-H

Purified by Chromatography, Storage buffer: PBS pH 7.2, 1% BSA, 0.05% NaN3

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

lactoferrin, partial; Lactotransferrin; lactotransferrin; kaliocin-1; lactoferricin; lactoferroxin; talalactoferrin; neutrophil lactoferrin; growth-inhibiting protein 12; epididymis luminal protein 110; lactotransferrin; Growth-inhibiting protein 12; TalalactoferrinCleaved into the following 5 chains:Lactoferricin-H; Lfcin-H; Kaliocin-1; Lactoferroxin-A; Lactoferroxin-B; Lactoferroxin-C

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

the antibody contains monoclonal antibody specific reagents in optimal concentrations of affinity-purified antibody. To enchance the antibody's stability sodium azide has been added. Keep the antibody at +4 degrees Celsius and do NOT freeze it. the antibody may be photosensitive, therefore avoid exposing the vial to direct bright light. Please refer to the expiry date printed on the vial. The use of the reagent after the expiration date is not recommended.

The LF mAb (clone 4C5) recognizes Lactoferrin stored within secondary granules of postmitotic granulocyte-committed cells. The sensitivity of 4C5 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity. ; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.