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- Anti-Mouse CD32/CD16, Biotin (Clone KT1632) (rat IgG2a)
Product short description
Price:
                                                                                    525 EUR
                                                                            
                                Size:
                                        0.3 miligrams
                                    
                                Catalog no.:
                                        GEN520093
                                    
                                Product detailed description
Other gene names
                                        N/A
                                    
                                Other names
                                        N/A
                                    
                                Purification method
                                        N/A
                                    
                                Gene name synonims
                                        N/A
                                    
                                Form/Appearance
                                        Biotin
                                    
                                Clone
                                        KT1632
                                    
                                Immunoglobulin isotype
                                        Rat IgG2a
                                    
                                Also known as
                                        CD32/CD16
                                    
                                Gene name
                                        CD32/CD16
                                    
                                Category
                                        Antibodies
                                    
                                Clonality
                                        Monoclonal
                                    
                                Conjugation
                                        Biotinylated
                                    
                                Subcategory
                                        Mnoclonal antibodies
                                    
                                Host organism
                                        Rat (Rattus norvegicus)
                                    
                                Tested applications:
                                        Immunohistochemistry (IHC)
                                    
                                Concentration
                                        0.1 miligram per 1 mililiter
                                    
                                Latin name
                                        Mus musculus,Rattus norvegicus
                                    
                                Description
                                        This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
                                    
                                Species reactivity
                                        Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
                                    
                                Storage and shipping
                                        For all formats, store the antibody at +4 degrees Celsius. For long term storage (Purified, Biotin, FITC), aliquot and freeze unused portion at - 20 degree C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
                                    
                                Specificity and cross-reactivity
                                        CD32/16 (Mouse CD32/16 Fcgamma III/II); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
                                    
                                About
                                        Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
                                    
                                Properties
                                        Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
                                    
                                Test
                                        Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
                                    
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