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- Anti-Mouse CD44, Biotin (Clone KM81) (rat IgG2a)
Product short description
Price:
398 EUR
Size:
0.3 miligrams
Catalog no.:
GEN520131
Product detailed description
Gene name synonims
N/A
Purification method
N/A
Gene name
CD44
Clone
KM81
Also known as
CD44
Immunoglobulin isotype
Rat IgG2a
Category
Antibodies
Clonality
Monoclonal
Conjugation
Biotinylated
Subcategory
Mnoclonal antibodies
Host organism
Rat (Rattus norvegicus)
Latin name
Mus musculus,Rattus norvegicus
Tested applications:
Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Concentration
Antibody Concentration: 0.1 miligram per 1 mililiter
Other gene names
CD44; CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III; LHR; MDU2; MDU3; MIC4; ECMR-III; PGP-1; PGP-I
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Storage and shipping
Store the antibody at +4 degrees Celsius. For long term storage, aliquot and freeze unused portion the antibody should be stored at -20 degrees Celsius. in volumes appropriate for single usage. Avoid freeze/thaw cycles.
Form/Appearance
Biotin (Biotin conjugated Ig buffered in PBS, 0.02% NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml. (Purified from bioreactor supernatant via Protein G Chromatography))
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Specificity and cross-reactivity
CD44 (Ly 24, Pgp-1) (Mouse CD44 (Pgp-1, Ly-24)); Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Other names
CD44; CD44 antigen; CD44 antigen; epican; Hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; heparan sulfate proteoglycan; cell surface glycoprotein CD44; extracellular matrix receptor III; chondroitin sulfate proteoglycan 8; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; homing function and Indian blood group system; CD44 molecule (Indian blood group); CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I
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