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- HAMSTER ANTI MOUSE CD49e:Biotin Antibody
Product short description
Price:
332 EUR
Size:
100ug
Catalog no.:
GEN210444
Product detailed description
Gene name synonims
N/A
Immunoglobulin isotype
IgG
Purification method
N/A
Also known as
CD49e
Gene name
CD49e
Host organism
Hamster
French translation
anticorps
Category
Antibodies
Clone
HM alpha 5
Clonality
Monoclonal
Conjugation
Biotinylated
Latin name
Mus musculus
Subcategory
Mnoclonal antibodies
Tested applications:
Flow Cytometry (FACS)
Other gene names
Itga5; Itga5; Fnra; VLA5; Cd49e; N/A
Concentration
IgG concentration 0.1 miligram per 1 mililiter
Form/Appearance
Biotin (Purified IgG conjugated to Biotin - liquid)
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
Other names
integrin alpha-5 preproprotein; Integrin alpha-5; integrin alpha-5; integrin alpha-F; CD49 antigen-like family member E; fibronectin receptor subunit alpha; fibronectin receptor alpha polypeptide; integrin alpha 5 (fibronectin receptor alpha); CD49 antigen-like family member E; Fibronectin receptor subunit alpha; Integrin alpha-F; VLA-5
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Specificity and cross-reactivity
CD49e This item recognises the mouse integrin alpha 5 subunit (CD49e). CD49e is a type I membrane protein that associates non-covalently with integrin beta 1 (CD29) to form the heterodimer CD49e/CD29 (VLA-5). VLA-5 is expressed on mast cells, splenic B cells, thymocytes, T-cells and monocytes. The VLA-5 heterodimer forms the fibronectin receptor, which plays an important role in cell adhesion and migration.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
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