MOUSE Anti-RAT CD11b:Biotin Antibody

359 EUR

100ug

GEN210479

N/A

N/A

N/A

N/A

CD11b

CD11b

IgG2a

OX-42

anticorps

Monoclonal

Antibodies

Biotinylated

Mouse (Mus musculus)

Mnoclonal antibodies

Mus musculus,Rattus norvegicus

IgG concentration 0.1 miligram per 1 mililiter

Biotin (Purified IgG conjugated to Biotin - liquid)

Immunohistology - Frozen (IHC), Flow Cytometry (FACS)

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

CD11b This item recognises the rat equivalent of human CD11b, the receptor for the iC3b component of complement. The antigen is expressed on most macrophages, including resident and activated peritoneal macrophages and Kupffer cells and around 35% of alveolar macrophages. The antibody also labels dendritic cells, granulocytes and microglial cells in the brain. Clone MRC OX-42 has been reported as being suitable for use on paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) Functionally, clone OX-42 inhibits complement mediated rosettes; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.