MOUSE Anti-RAT CD43:Biotin Antibody

359 EUR

100 Tests

GEN210502

N/A

N/A

CD43

CD43

IgG1

W3/13

anticorps

Antibodies

Monoclonal

Biotinylated

Mnoclonal antibodies

Mouse (Mus musculus)

Flow Cytometry (FACS)

IgG concentration 0.23 mg/ml

Mus musculus,Rattus norvegicus

Spn; Spn; Lsn; Cd43; Lsn1; gpL115; N/A

Biotin (Purified IgG conjugated to Biotin - lyophilised)

Leukosialin; Leukosialin; leukosialin; leukosianin; W3/13 antigen; leukocyte sialoglycoprotein; sialophorin; Leukocyte sialoglycoprotein; Sialophorin; W3/13 antigen

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

CD43 This item recognises the rat CD43 cell surface antigen, also known as leukosialin and sialophorin. CD43 is a 95kD heavily glycosylated glycoprotein expressed by all leucocytes with the exception of B lymphocytes. _x000D__x000D_This product is routinely tested in flow cytometry on rat splenocytes.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

Prior to reconstitution Keep the antibody refrigerated at +4 degrees Celsius. Temperature variations in the range between +1C to +7C are tolerable. Following reconstitution Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 12 months from date of reconstitution.