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- RAT ANTI HUMAN CD28:Biotin Antibody
Product short description
Price:
475 EUR
Size:
100ug
Catalog no.:
GEN210509
Product detailed description
Gene name synonims
N/A
Purification method
N/A
Also known as
CD28
Gene name
CD28
Immunoglobulin isotype
IgG2b
Clone
YTH913.12
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Conjugation
Biotinylated
Latin name
Rattus norvegicus
Subcategory
Mnoclonal antibodies
Tested applications:
Flow Cytometry (FACS)
Other gene names
CD28; CD28; Tp44; N/A
Host organism
Rat (Rattus norvegicus)
Concentration
Ig concentration 0.1 miligram per 1 mililiter
Form/Appearance
Biotin (Purified IgG conjugated to Biotin - liquid)
Other names
T-cell-specific surface glycoprotein CD28 isoform 2; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; CD28 antigen; CD28 molecule; TP44
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Specificity and cross-reactivity
CD28 This item recognises the human CD28 cell surface antigen, a 44kD glycoprotein expressed by T lymphocytes. It has been reported that this clone enhances the MLR response Clone YTH913.12 recognizes an epitope of CD28 expressed by NK cells, which is not recognised by clones of 9.3 or CD28.2 For functional studies,; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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