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- RAT ANTI MOUSE CD107b:FITC Antibody
Product short description
Price:
277 EUR
Size:
100ug
Catalog no.:
GEN210923
Product detailed description
Gene name synonims
N/A
Purification method
N/A
Immunoglobulin isotype
IgG1
Clone
M3/84
Also known as
CD107b
Gene name
CD107b
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Tested applications:
Flow Cytometry (FACS)
Host organism
Rat (Rattus norvegicus)
Latin name
Mus musculus,Rattus norvegicus
Concentration
IgG concentration 0.1 miligram per 1 mililiter
Form/Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
Other gene names
Lamp2; Lamp2; Mac3; CD107b; Lamp-2; Lamp-2a; Lamp-2b; Lamp-2c; Lamp-2; LAMP-2; Lysosome-associated membrane protein 2; LGP-B
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Other names
lysosome-associated membrane glycoprotein 2 isoform 1; Lysosome-associated membrane glycoprotein 2; lysosome-associated membrane glycoprotein 2; LGP-B; Lamp II; lysosomal membrane glycoprotein 2; CD107 antigen-like family member B; lysosomal membrane glycoprotein type B; lysosome-associated membrane protein 2; lysosome-associated membrane protein-2; lysosomal-associated membrane protein 2; CD107 antigen-like family member B; Lysosomal membrane glycoprotein type B
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Specificity and cross-reactivity
CD107b This item recognises murine CD107b, also known as MAC-3 and LAMP-2. CD107b is a transmembrane glycoprotein that is associated with lysosomal membranes and is primarily expressed on mononuclear phagocytes. Expression of CD107b does vary between cell populations and the molecular weight of CD107b can vary between 92-120kD. Studies suggest that CD107b may be involved in leucocyte adhesion. Please note: the expression of CD107b is predominantly cytoplasmic - flow cytometry results are improved by the use of a membrane permeabilisation procedure prior to staining.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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