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- RAT ANTI HUMAN HLA DR:FITC Antibody
Product short description
Price:
448 EUR
Size:
100ug
Catalog no.:
GEN211077
Product detailed description
Gene name synonims
N/A
Other gene names
N/A
Other names
N/A
Purification method
N/A
Immunoglobulin isotype
IgG2a
Gene name
HLA DR
Also known as
HLA DR
French translation
anticorps
Clone
YE2/36-HLK
Clonality
Monoclonal
Category
Antibodies
Latin name
Rattus norvegicus
Conjugation
Anti-FITC Antibody
Subcategory
Mnoclonal antibodies
Tested applications:
Flow Cytometry (FACS)
Host organism
Rat (Rattus norvegicus)
Concentration
IgG concentration 0.1 miligram per 1 mililiter
Form/Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Specificity and cross-reactivity
HLA DR The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In humans, this complex is referred to as the human leukocyte antigen (HLA) region. There are 3 major MHC class II proteins encoded by the HLA which are HLA DP, HLA DQ and HLA DR. This item recognises a monomorphic determinant of human HLA DR which is an MHC class II cell surface receptor expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages and activated T lymphocytes. Clone YE2/36 has also been reported to be cross reactive with mouse H-2 haplotypes b, d and q expressed by mouse strains including C57BL/6, DBA, and NZB; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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