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- MOUSE Anti-RAT TCR ALPHA/BETA:Low Endotoxin Antibody
Product short description
Price:
608 EUR
Size:
500ug
Catalog no.:
GEN211259
Product detailed description
Gene name synonims
N/A
Other gene names
N/A
Clone
R73
Other names
N/A
Purification method
N/A
Immunoglobulin isotype
IgG1
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Gene name
TCR ALPHA/BETA
Also known as
TCR ALPHA/BETA
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Concentration
IgG concentration 1.0 mg/ml
Latin name
Mus musculus,Rattus norvegicus
Form/Appearance
Low Endotoxin (Purified IgG - liquid)
Tested applications:
Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistology - Frozen (IHC), Functional Assay (FA)
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Species reactivity
Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Description
The MOUSE Anti- TCR ALPHA/BETA: Endotoxin Antibody is a α- or alpha protein sometimes glycoprotein present in blood.This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Storage and shipping
Store at -20 degree only. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Specificity and cross-reactivity
TCR ALPHA/BETA This item recognises a constant determinant on the beta chain of the rat alpha/beta T cell receptor, expressed by 97% of peripheral rat T cells as defined by the OX-52 marker. Clone R73 is mitogenic for unseparated spleen cells and for purified T cells. In the rat thymus, mature medullary cells express the R73 determinant at the same levels as peripheral T cells, whereas 94% of CD4 - CD8 - thymocytes are R73 negative. This clone is reported to stimulate adhesion between Thymic Dendritic Cells and Thymocytes; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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