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- MBS Monoclonals
- MOUSE Anti-RABBIT CD43 Antibody
Product short description
Price:
564 EUR
Size:
2 ml
Catalog no.:
GEN214195
Product detailed description
Gene name synonims
N/A
Other gene names
N/A
Other names
N/A
Purification method
N/A
Also known as
CD43
Gene name
CD43
Immunoglobulin isotype
IgG1
Clone
L11/135
French translation
anticorps
Clonality
Monoclonal
Category
Antibodies
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Latin name
Mus musculus,Oryctolagus cuniculus
Concentration
Total protein concentration 16.7 mg/ml
Form/Appearance
S/N (Tissue Culture Supernatant - liquid)
Tested applications:
Immunohistology - Paraffin (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistology - Frozen (IHC), Immunoprecipitation (IP)
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Rabbit (Oryctolagus cuniculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
About
Rabbits are used for polyclonal antibody production by MBS Monoclonals. Rabbit antibodies are very stable and can be stored for several days at room temperature. MBS Monoclonals adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Specificity and cross-reactivity
CD43 This item recognises all T cells by flow cytometry; monocytes and macrophages stain weakly. Good staining of T-Cell dependent areas in lymphoid tissue sections is observed. This antibody immunoprecipitates and immunoblots a 120kD protein from RL-5 cells and T lymphocytes. It is believed to recognise the homologue of Human CD43, Leukosialin. Clone L11/135 has been reported as being suitable for use in Western blotting.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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