RAT ANTI MOUSE Ly-6A/E:Biotin Antibody

299 EUR

100ug

GEN214528

D7

N/A

N/A

IgG2a

Ly-6A/E

Ly-6A/E

anticorps

Antibodies

Monoclonal

Biotinylated

Mnoclonal antibodies

Rat (Rattus norvegicus)

IgG concentration 500ug/1000ul

Mus musculus,Rattus norvegicus

Biotin (Purified IgG conjugated to Biotin - liquid)

Ly6a; Ly6a; TAP; Sca1; Sca-1; Ly-6A.2; Ly-6A/E; Ly-6E.1; Ly6; Ly-6A.2/Ly-6E.1; SCA-1; TAP

Immunohistology - Frozen (IHC), Flow Cytometry (FACS), Western Blot (WB), Immunoprecipitation (IP)

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.

lymphocyte antigen 6A-2/6E-1; Lymphocyte antigen 6A-2/6E-1; lymphocyte antigen 6A-2/6E-1; stem cell antigen 1; T-cell-activating protein; lymphocyte antigen 6 complex, locus A; Stem cell antigen 1; SCA-1; T-cell-activating protein

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

Ly-6A/E This item recognises members of the lymphocyte activation protein-6 (Ly-6) multigene family. Specifically, it recognises Ly-6A.2 and Ly-6E.1. Ly6-A/E is also known as stem cell antigen-1 (Sca-1). It is an 18kDa mouse glycosyl phosphatidylinositol-anchored cell surface protein of the Ly-6 gene family. It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes. As such, it is the most common marker used for enriching adult hematopoietic stem cells. It has varied functions, it plays a role in bone formation, myoblast proliferation and differentiation and the activation and immunosuppressive function of T cells. _x000D__x000D_Clone D7 (MBS214527) has been reported to induce T cell activation.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.