MOUSE Anti-DOG CD4 Antibody

597 EUR

2 ml

GEN215436

CD4

CD4

N/A

N/A

N/A

IgG1

CA13.1E4

anticorps

Monoclonal

Antibodies

Mus musculus

CD4; CD4; N/A; N/A

Mouse (Mus musculus)

Mnoclonal antibodies

S/N (Tissue Culture Supernatant - liquid)

Immunoprecipitation (IP), Immunohistology - Frozen (IHC), Flow Cytometry (FACS)

Dog; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4; CD4 antigen (p55); T-cell surface antigen T4/Leu-3; N/A; T-cell surface antigen T4/Leu-3

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Canine or Canis Lupus is mostly Beagle used for drug research. MBS Monoclonals produces ELISA test kits and polyclonal antibodies.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.CD4+ T helper cell marker or cluster differentiator of white blood cells in FACS or flow cytometry. Used offer coupled to magnetic beads. GENTAUR makes custom magnetic bead couplings on 50 and 250 nm beads for certain clones.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

CD4 Mouse anti Dog CD4 antibody (CA13.1E4), a monoclonal antibody specific for canine CD4. CA13.1E4 was clustered at the first Canine Leukocyte Antigen Workshop (Claw) and identifies, by immunoprecipitation a 60kDa monomeric protein under both reducing and non-reducing conditions. Canine CD4 is a surface glycoprotein expressed by non CD8 expressing T lymphocytes, with similar developmental and functional characteristics to T helper cells described in other mammalian species. Mouse anti Dog CD4 also recognizes a population of CD8 positive cells in the canine thymus. CD4 expression has also been reported on a population of CD3 positive peripheral blood T lymphocytes which are double positive for CD4 and CD8. Similar reports have been made for cynomolgus monkey, pig as well as rat, chicken and human (reviewed). _x000D_Uniquely amongst mammalian species clone CA13.1E4 also recognizes CD4 expressed on canine neutrophils at a similar density to that expressed on canine T helper cells. The functional significance of this is not understood and remains enigmatic in view of the roles described for CD4 in canine and other mammalian species; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.