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- MOUSE Anti-CHICKEN CD8a:FITC Antibody
Product short description
Price:
647 EUR
Size:
100ug
Catalog no.:
GEN215565
Product detailed description
Gene name synonims
N/A
Other gene names
N/A
Other names
N/A
Purification method
N/A
Also known as
CD8a
Gene name
CD8a
Immunoglobulin isotype
IgG1
Clone
14550
French translation
anticorps
Clonality
Monoclonal
Category
Antibodies
Latin name
Mus musculus
Conjugation
Anti-FITC Antibody
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Tested applications:
Flow Cytometry (FACS)
Form/Appearance
FITC (Purified IgG - liquid)
Concentration
IgG concentration 0.1 miligram per 1 mililiter
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Chicken; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Specificity and cross-reactivity
CD8a This item recognises the alpha chain of the chicken CD8 homologue, a 33-35kD cell surface protein. CD8 is expressed as either alpha/alpha homodimers or alpha/beta heterodimers on a subpopulation of T cells and NK cells. Clone 11-39 recognises all polymorphic forms of chicken CD8 alpha. Clone 11-39 has been demonstrated to recognise Turkey CD8 alpha.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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