MOUSE Anti-MOUSE MHC CLASS I:Biotin Antibody

503 EUR

100ug

GEN215605

N/A

N/A

2G5

N/A

N/A

IgG2b

anticorps

Antibodies

Monoclonal

MHC CLASS I

MHC CLASS I

Biotinylated

Mus musculus

Mouse (Mus musculus)

Mnoclonal antibodies

Flow Cytometry (FACS)

IgG concentration 0.1 miligram per 1 mililiter

Biotin (Purified IgG conjugated to Biotin - liquid)

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

MHC CLASS I The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. This item recognises a monomorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells. The epitope recognised by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this antibody may recognise a polymorphic epitope of canine MHC class I.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.