RAT ANTI MOUSE PLVAP:Biotin Antibody

271 EUR

100ug

GEN216239

N/A

N/A

PLVAP

PLVAP

IgG2a

MECA-32

anticorps

Antibodies

Monoclonal

Biotinylated

Mnoclonal antibodies

Rat (Rattus norvegicus)

Mus musculus,Rattus norvegicus

Plvap; Plvap; Pv1; MECA32; Pv1; PV-1

IgG concentration 0.1 miligram per 1 mililiter

Biotin (Purified IgG conjugated to Biotin - liquid)

Flow Cytometry (FACS), Immunohistology - Frozen (IHC)

Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

plasmalemma vesicle-associated protein; Plasmalemma vesicle-associated protein; plasmalemma vesicle-associated protein; MECA-32 antigen; plasmalemma vesicle protein 1; plasmalemma vesicle associated protein; MECA-32 antigen; Plasmalemma vesicle protein 1

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

PLVAP This item recognises murine plasmalemma vesicle-associated protein (PLVAP), a 60kD protein which is otherwise known as MECA-32 and PV-1. PLVAP is a transmembrane homodimer which is associated with endothelial cell caveolae and fenestrae. In adult and embryonic mice, PLVAP is primarily expressed on endothelial tissues but shows restricted distribution in the skeletal, cardiac and brain tissues. In embryonic mice, PLVAP expression, on the vasculature associated with blood brain barrier has been reported to be developmentally regulated. _x000D__x000D_In humans, PLVAP is selectively up-regulated in a variety of high-grade brain tumors, including glioblastoma and metastatic carcinoma, as well as other cerebral disorders associated with blood-brain barrier disruption, such as acute ischemia. PLVAP represents a novel marker of brain tumour angiogenesis and integrity of the blood-brain barrier and is a potential therapeutic target.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.