MOUSE Anti-MHC CLASS II H-2I-Ak/s:FITC Antibody

332 EUR

100ug

GEN216289

N/A

N/A

N/A

N/A

N/A

IgG1

OX-6

anticorps

Antibodies

Monoclonal

Mus musculus

Anti-FITC Antibody

Mnoclonal antibodies

Mouse (Mus musculus)

Flow Cytometry (FACS)

MHC CLASS II H-2I-Ak/s

IgG concentration 0.1 miligram per 1 mililiter

FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term. the antibody should be stored undiluted. Storage in frost free freezers is not recommended. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.

MHC CLASS II H-2I-Ak/s The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the H-2 region. In rats, this complex is referred to as the RT1 region. MBS214438 recognises a monomorphic determinant of the rat RT1B MHC class II antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. The literature reports that this clone does not react with the rat BDIX strain due to a defect in RT1B expression This item also cross reacts with a polymorphic determinant on mouse strains of the H-2 haplotypes k and s. Analysis of recombinant mouse strains has mapped the OX-6 determinant to the H-2I-A region This product is routinely tested in flow cytometry on rat splenocytes.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.