MOUSE Anti-RABBIT T LYMPHOCYTES:FITC Antibody

647 EUR

100 Tests

GEN216607

N/A

N/A

N/A

N/A

N/A

IgG1

KEN-5

anticorps

Monoclonal

Antibodies

T LYMPHOCYTES

Anti-FITC Antibody

Mouse (Mus musculus)

Mnoclonal antibodies

Flow Cytometry (FACS)

Mus musculus,Oryctolagus cuniculus

IgG concentration 0.1 miligram per 1 mililiter

FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Rabbit (Oryctolagus cuniculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Rabbits are used for polyclonal antibody production by MBS Monoclonals. Rabbit antibodies are very stable and can be stored for several days at room temperature. MBS Monoclonals adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.

Store the antibody at +4 degrees Celsius. for one month or the antibody should be stored at -20 degrees Celsius. for longer. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

T LYMPHOCYTES This item recognises rabbit T lymphocytes and immunoprecipitates, a 67kD glycoprotein. This antibody was initially reported as recognising rabbit CD5, but does not bind to rabbit CD5 transfectants. Known rabbit CD5 antibodies also show binding to most B lymphocytes, which are not labelled by this clone.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.