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- HAMSTER ANTI MOUSE DELTA-LIKE PROTEIN 4 Antibody
Product short description
Price:
277 EUR
Size:
100ug
Catalog no.:
GEN219862
Product detailed description
Immunoglobulin isotype
IgG
Purification method
N/A
Clone
HMD4-2
Host organism
Hamster
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Latin name
Mus musculus
Subcategory
Mnoclonal antibodies
Also known as
DELTA-LIKE PROTEIN 4
Concentration
IgG concentration 1.0mg/ml
Other gene names
Dll4; Dll4; Delta4; Delta4
Gene name synonims
Dll4; Dll4; Delta4; Delta4
Gene name
Dll4; Dll4; Delta4; Delta4
Form/Appearance
Purified (Purified IgG - liquid)
Tested applications:
Flow Cytometry (FACS), Functional Assay (FA), Immunohistology - Paraffin (IHC), Immunohistology - Frozen (IHC)
Other names
delta-like protein 4; Delta-like protein 4; delta-like protein 4; drosophila Delta homolog 4; delta-like 4 (Drosophila); Drosophila Delta homolog 4
Species reactivity
Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Armenian of Syrian hamster monoclonal IgGs are useful antibodies for double staining your IHC samples. Using mabs or monoclonal antibodies has a lot of advantages for Flow cytometry.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Specificity and cross-reactivity
DELTA-LIKE PROTEIN 4 This item specifically recognises mouse Delta-like protein 4 (DLL4), one of the five major ligands of the Notch signalling pathway, which is activated through the binding of specific ligands to the Notch receptors Notch 1-4. _x000D__x000D_The Notch signalling pathway is an evolutionarily conserved pathway in multi-cellular organisms, which is vital for cell-cell communication, important during fundamental developmental and physiological processes, including regulation of cell fate decisions during neuronal, cardiac and endocrine development, stem cell haematopoiesis, thymic T-cell development, and both tumour progression and suppression. _x000D__x000D_Ligation of Notch receptors by their specific ligands, Jagged1 (CD339), Jagged2, Delta like-1 (DLL1), DLL3 and DLL4, on physically adjacent signal receiving cells, induces proteolysis of the receptors by ADAM-family metalloproteases and gamma-secretase complex, within the transmembrane domain, releasing the Notch intracellular domain (NICD) to translocate to the nucleus. Subsequent signal transduction then occurs through either the CSL-NICD-Mastermind complex cascade (canonical pathway), or NF-kappaB-NICD and CSL-NICD-Deltex complex signalling cascades (non-canonical pathway). The canonical pathway inhibits the differentiation of stem cells or progenitor cells, whilst the non-canonical pathway promotes differentiation. _x000D__x000D_DLL4 is expressed by vascular endothelium, and plays a vital role in embryonic vascular development. DLL4 signalling has been shown to play a role in the angiogenesis of clear-cell renal tumours, and pancreatic, bladder and colonic cancer. Studies have shown that DLL4 expression in endothelium cells, can be up-regulated by vascular endothelial growth factor (VEGF) and basic-FGF, and by HIF1 alpha, and that blockade of DLL4 inhibits tumour growth by promoting non-productive angiogenesis. _x000D_Clone HMD4-2 has been shown to block mouse Notch1-Fc and Notch4-Fc binding to mou; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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