- Our Suppliers
- MBS Monoclonals
- MOUSE Anti-HORSE CD44:FITC Antibody
Product short description
Price:
647 EUR
Size:
100ug
Catalog no.:
GEN224468
Product detailed description
Gene name synonims
N/A
Purification method
N/A
Also known as
CD44
Gene name
CD44
Immunoglobulin isotype
IgG1
Clone
CVS18
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Latin name
Mus musculus
Conjugation
Anti-FITC Antibody
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Tested applications:
Flow Cytometry (FACS)
Other gene names
CD44; CD44; N/A; ECMR-III; PGP-1; PGP-I
Concentration
IgG concentration 0.1 miligram per 1 mililiter
Form/Appearance
FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Species reactivity
Horse; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Storage and shipping
Store the antibody at +4 degrees Celsius. for one month or the antibody should be stored at -20 degrees Celsius. for longer. the antibody should be stored undiluted. Storage in frost-free freezers is not recommended. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
Other names
CD44 antigen; CD44 antigen; CD44 antigen; PGP-1; PGP-I; HUTCH-I; ECMR-III; hermes antigen; hyaluronate receptor; phagocytic glycoprotein 1; phagocytic glycoprotein I; extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; lymphocyte surface antigen precursor CD44; N/A; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I
Specificity and cross-reactivity
CD44 Clone CVS18 specifically recognises equine CD44, a plasma membrane glycoprotein broadly expressed on the cell surface of leucocytes. CD44 is the primary receptor for hyaluronate and functions in cell adhesion. Equine CD44 is widely expressed and the CVS18 antibody may be used as a pan equine leucocyte marker.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.Horse (Equus ferus caballus) sera and plasma contain equine IgGs, Immunoglobulins. ELISA test are used to determine quantitatively the presence in horse serum of the antigen by a polyclonal antibody to the equine epitope selected for the ELISA kit. A blocking solution for the native horse or equine immunoglobulins in available in the ELISA protocol.
© Copyright 2016-Tech News . Design by: uiCookies
