MOUSE Anti-RAT CD252:FITC Antibody

243 EUR

0.025 miligrams

GEN224536

N/A

N/A

IgG1

CD252

CD252

ATM-2

anticorps

Antibodies

Monoclonal

Anti-FITC Antibody

Mnoclonal antibodies

Mouse (Mus musculus)

Flow Cytometry (FACS)

Mus musculus,Rattus norvegicus

Tnfsf4; Tnfsf4; Ox40l; Ox40l; Txgp1; OX40L

IgG concentration 0.1 miligram per 1 mililiter

FITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

tumor necrosis factor ligand superfamily member 4; Tumor necrosis factor ligand superfamily member 4; tumor necrosis factor ligand superfamily member 4; OX40 ligand; tumor necrosis factor (ligand) superfamily, member 4; OX40 ligand

Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.

CD252 This item specifically recognises rat CD252, otherwise known as OX40L or CD134L, a 22kDa type II transmembrane glycoprotein and member of the TNF superfamily, expressed by antigen-presenting cells including activated dendritic cells and B cells, and also by vascular endothelium. _x000D__x000D_The interaction of CD252 with its receptor OX40/CD134, acts as a co-stimulator of T cell activation, proliferation and both Th1 and Th2 cytokine production, and is also important in activated B cell proliferation and secretion of immunoglobulins (Igs). CD252/OX40 interaction has been implicated in HTLV-1 associated diseases including myelopathy and adult T cell leukaemia, and also with the autoimmune diseases rheumatoid arthritis and inflammatory bowel disease. _x000D__x000D_Clone ATM-2 has been shown to block the co-stimulatory activity of CD252 on rat T cell proliferation.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.